Fig. 5.

Hydrogen-deuterium exchange dynamics. HDX of αVβ8 and αVβ6 headpiece fragments in presence or absence of TGF-β1 ligand peptide at 1 min. (a–d). Close-ups of the βI domain around the ligand-binding site. (e–h). The β-propeller, hybrid, and βI domains. Cartoon diagrams are colored according to the key for deuterium exchange at 1 min. for a single set of non-overlapping peptides (a-d) or for all peptides (e-h), except TGF-β1 peptide is shown in magenta. As HDX data covers regions disordered in crystal structures, the structure of SDL2 from liganded αVβ8 and the hybrid domain from αVβ6 are used to model disordered regions of αVβ8; furthermore, the hybrid domain of αVβ6 swings out in presence of ligand and its position is modeled on αIIbβ3.