Fig. 4.

DUB3 is in a complex with NRF2 and KEAP1. a NRF2 is colocalized with DUB3. U2OS cells were cotransfected with NRF2-Myc and DUB3-Flag plasmids. Fluorescence confocal microscopy analysis was performed 36 h after transfection. b NRF2 interacts with DUB3. HEK293T cells were transfected with the indicated plasmids. Anti-HA or anti-Flag immunoprecipitates were immunoblotted with anti-HA (NRF2) or anti-Flag (DUB3) antibodies, respectively. c KEAP1 interacts with DUB3. The experiments were performed as for panel (b). d An endogenous interaction between NRF2, DUB3, and KEAP1 is detected. Anti-NRF2 or anti-DUB3 immunoprecipitates were immunoblotted with NRF2, DUB3 or KEAP1 antibodies, respectively, in HCT116 cells. e Schema chart for a complex containing DUB3, NRF2, and KEAP1