Figure 6.

(A–C) Rapid kinetics of FPR binding to the thrombin mutants I16, D194A and the zymogen precursor prethrombin-2, all carrying the S195A substitution, over the temperature range 5–30 °C. Continuous lines were drawn according to Eq. 4 in the text with each rate constant expressed as in Eq. 5 and represent a global fit of the entire data set with best-fit parameter values listed in Table 3. The values of the rate constants are at the reference temperature T₀ = 288.15 K (15 °C). Experimental conditions are: 400 mM ChCl, 50 mM Tris, 0.1% PEG8000, pH 8.0 at 5 °C (closed circles), 10 °C (open circles), 15 °C (closed squares), 20 °C (open squares), 25 °C (closed triangles), 30 °C (open triangles).