Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Hydrolysis of natural substrates and Na+ binding.
| Fibrinogen kcat/Km (μM−1 s−1) | PAR1 kcat/Km (μM−1 s−1) | Protein C1 kcat/Km (mM−1 s−1) | Na+ Kd (mM) | |
|---|---|---|---|---|
| wt | 17 ± 1 | 32 ± 2 | 220 ± 10 | 16 ± 3 |
| I16T | 0.021 ± 0.001 | 0.034 ± 0.001 | 7.3 ± 0.1 | 1100 ± 300 |
| D194A | 0.0052 ± 0.0003 | 0.0059 ± 0.0002 | 0.15 ± 0.01 | 80 ± 20 |
1In the presence of 50 nM rabbit thrombomodulin. Experimental conditions described under Materials.
