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. 2019 Dec 2;9:18072. doi: 10.1038/s41598-019-54061-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Area stretch ratio of the cardiac walls at (A) mid-ventricular-systole and (B) mid-ventricular diastole for the 5 embryonic subjects evaluated. Circled area showed the appendages. Area stretch of 1.0 indicated no contractile stretch, while area stretch of less than 1.0 indicates contraction. A video of area stretch for case 2 was uploaded as online Supplementary Video 1.