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. 2019 Dec 2;9:18072. doi: 10.1038/s41598-019-54061-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Averaged WSS magnitude of different heart structures across a cardiac cycle and at atrioventricular junction for 5 embryos. (B–E) Surface WSS plot of embryonic heart. Higher WSS were noticed at region with narrow area such as the inlet, atrioventricular junction, outlet, middle region of atria, and mid region of ventricle. (B,C) Ventricle mid-systole, maximum flow at ventricle outlet. (D,E) Ventricle mid-diastole, maximum flow at ventricle inlet (C,E) dorsal view. (B,D) ventral view.