Fig. 8.

Identification of the Sirt6-mediated deacetylation sites of ERα. a HEK293T cells were transfected with Era and p300 with or without Sirt6. Acetylation of ERα was determined using LC-MS/MS analysis after in-gel digestion. MS/MS spectra of acetylated ERα peptides containing Lys171 (left) and Lys299 (right) are shown. b HEK293T cells transfected with wild (WT) or mutant Era (K171R or K299R) were treated with E2 (10 nM) for the indicated time. The relative protein levels of ERα were compared (n = 4). c HEK293T cells were transfected with WT or mutant Era, and FasL luciferase activity was determined (n = 4). The values are mean ± SEM. *p < 0.05 and **p < 0.01 versus WT