Table 2.
Nucleotide diversity in dhfr and dhps allelic loci in sub-population areas with their different test parameters
| No. of samples (n) | dhfr | dhps | ||||
|---|---|---|---|---|---|---|
| Ranchi | Raipur | Mewat | Ranchi | Raipur | Mewat | |
| 22 | 40 | 60 | 22 | 40 | 60 | |
| Mutant (%) | 16 (72.70) | 25 (62.50) | 32 (53.30) | 18 (81.80) | 24 (60) | 31(51.60) |
| SNP (n) total number of mutant alleles found in different regions | 27 | 46 | 52 | 38 | 41 | 50 |
| Nucleotide diversity: Ɵ | 1.09 | 0.9 | 0.8 | 1.09 | 0.4 | 1.07 |
| Pairwise difference: π | 1.13 | 1.11 | 1.35 | 0.8 | 1.02 | 1.31 |
| Test of neutrality | ||||||
| Tajima’s D | 0.094473 | 0.437752 | 1.276397 | − 0.747549 | 2.225384 | 0.526865 |
| Fu and Li’s D* | 0.142505 | − 0.07277 | 0.986851 | − 0.81047 | 0.771237 | 1.080688 |
| Fu and Li F* | 0.137096 | − 0.698455 | 1.197038 | − 0.842395 | 1.297203 | 1.00836 |
Tajima’s D, Fu, Li’s D* and Li F* test used for standardized measure of average number of mutations between pairs in the sample. Nucleotide diversity (Ɵ) and pairwise difference (π) indicates the distribution of mutation rate which varies among the regions. # Total number of field isolates in the parenthesis for that particular region. P value for all the three sites for the two genes was found to be non-significant (< 0.05)