Table 1.

Functional evaluation of ItfWRKYs under abiotic stress

Function known WRKYs related abiotic stress				Homologous WRKYs in I. trifida
Species	Protein name	Function	Reference	Protein name	Protein identify (%)	Gene expression under abiotic stress
Arabidopsis	AtWRKY25	Overexpression enhanced heat resistance and confer salt tolerance	[14]	ItfWRKY28.1	50.59	Under heat ↑ Under salt ↑
	AtWRKY26	Overexpression enhanced heat resistance	[14]	ItfWRKY43.1	51.69	Under heat ↑
	AtWRKY33	High-temperature represses its expression and induces the expression of AtWRKY25 and AtWRKY26; and confer salt tolerance	[14]	ItfWRKY13.3	52.99	Under heat ↓ Under salt ↑
	AtWRKY34	Negative regulator in pollen specific cold response	[16]	ItfWRKY38	49.34	Under cold ↑
	AtWRKY39	Overexpression enhanced heat stress resistance of transgenic plants	[17]	ItfWRKY44.2	51.30	Under heat ↑
	AtWRKY57	Induced by drought and its expression increase Arabidopsis drought tolerance	[18]	ItfWRKY4	84.93	Under drought ↑
Triticum aestivum	TaWRKY1	Confer drought and/or heat resistance in Arabidopsis	[18]	ItfWRKY65	64.00	Under drought ↓ Under heat ↑
	TaWRKY2	Confer transgenic plants tolerance to drought stress	[69]	ItfWRKY13.1 ItfWRKY13.3	49.49 49.49	Under drought ↓ Under drought ↓
	TaWRKY10	Overexpression enhanced the tobacco drought and salt tolerance	[21, 23]	ItfWRKY41	65.17	Under salt ↑ Under drought ↑
	TaWRKY19	Confer transgenic plants tolerance to drought stress	[69]	ItfWRKY79	48.08	Under drought ↑
	TaWRKY33	Confer drought and/or heat resistance in Arabidopsis	[18]	ItfWRKY67	41.43	Under drought ↑ Under heat ↑
	TaWRKY44	Confers transgenic tobacco multiple abiotic stress tolerances.	[70]	ItfWRKY79	50.44	Under salt ↑ Under drought ↑ Under heat ↑ Under cold ↑
Oryza.sativa L	OsWRKY11	Transgenic lines showed significant heat and drought tolerance	[71]	ItfWRKY64	78.16	Under heat ↓ Under drought ↑
	OsWRKY45	Overexpression enhances salt and drought tolerance	[19, 20]	ItfWRKY70	48.61	Under salt ↓ Under drought ↓
	OsWRKY72	Overexpression enhances salt and drought tolerance	[19, 20]	ItfWRKY77	86.96	Under salt ↑ Under drought ↑
Glycine max	GmWRKY13	Transgenic plants increased sensitivity to salt and drought stress while decreased sensitivity to ABA	[28]	ItfWRKY46	63.89	Under salt ↑ Under drought ↑
	GmWRKY21	Confer transgenic plant tolerance to cold stress	[28]	ItfWRKY41	66.22	Under cold ↑
	GmWRKY27	Improved salt and drought tolerance by inhibiting expression of downstream gene GmNAC29	[18, 72]	ItfWRKY15.2	53.74	Under salt ↑ Under drought ↓
	GmWRKY54	Confer transgenic Arabidopsis plants tolerance to salt and drought	[28]	ItfWRKY64	75.61	Under salt ↑ Under drought ↑
Gossypium hirsutum	GhWRKY17	Overexpression increases Nicotiana tolerance to drought and salt stress	[24]	ItfWRKY30	63.49	Under drought ↓ Under salt ↓
	GhWRKY25	Overexpression plants increased salt tolerance but reduced drought tolerance of A. thaliana	[26]	ItfWRKY19	78.57	Under salt ↑ Under drought ↓
	GhWRKY68	Reduces resistance to salt and drought in transgenic Nicotiana benthamiana	[75]	ItfWRKY64	75.58	Under salt ↑ Under drought ↑
Dendranthma grandiflorum	DgWRKY1	Overexpression enhances salt tolerance	[74]	ItfWRKY77	85.56	Under salt ↑
	DgWRKY3	Overexpression enhances salt tolerance	[74]	ItfWRKY70	65.59	Under salt ↓
Boea hygrometria	BhWRKY1	Bind to BhGolS1 to activate the regulation of BhGolS1 under drought stress	[27]	ItfWRKY15.1	56.52	Under drought ↑
Vitis vinifera	VvWRKY24	Be induced by cold treatment at all-time points	[15]	ItfWRKY49	76.32	Under cold ↑
Zea mays	ZmWRKY23	Enhance tolerance to salt stress	[25]	ItfWRKY72	50.00	Under salt ↓
Brassica campestris ssp	BcWRKY46	Overexpression increases A. thaliana tolerance to drought and salt stress	[73]	ItfWRKY30	55.74	Under salt ↓ Under drought ↓

↓ indicated decrease of gene expression level; ↑ indicated increase of gene expression level