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. 2019 Oct 31;21:603–611. doi: 10.1016/j.isci.2019.10.054

Figure 2.

Figure 2

Sporozoite Entry Is Associated with LE/lysosome Redistribution

(A) Hepa1-6 cells were infected with P. yoelii sporozoites and fixed after 30 or 90 min. DNA was visualized with DAPI (blue), actin with phalloidin (white), LE/Lysosomes with anti-LAMP1 (red), and parasites with anti-CSP (green). Images are maximum intensity projections of the 3D dataset. Scale bar represents 5 μm. The isospots corresponding to lysosomes away from the nucleus are cyan while perinuclear lysosomes are shown in magenta.

(B and C) Values represented in box and whiskers plots correspond to lysosomes from total and perinuclear area represented as mean ± SD of 25 different microscopic fields from three independent experiments. *p < 0.001.

(D) Hepa1-6 cells were infected with P. yoelii sporozoites for 90 min and analyzed by flow cytometry using antibodies specific to LAMP1 and CSP. The histogram depicts the distribution of total and surface LAMP1 from infected, uninfected, and unexposed control cells from one representative experiment.

(E) Total and surface LAMP1 levels were compared between uninfected and infected cells as a fold change over control cells. The bar graph depicts the mean ± the SD of three independent experiments. *p < 0.001.