Figure 3.

The Role of Lysosome Exocytosis Varies across Species Of Intracellular Parasites

(A and B) Hepa1-6 cells were infected with P. yoelii sporozoites, T. gondii tachyzoites or T. cruzi trypomastigotes for 90 min and analyzed by flow cytometry. The histogram shows surface LAMP1 from infected, uninfected, and unexposed control cells. Surface LAMP1 is expressed as fold change over uninfected cells. The bar graph displays the mean ± SD of three independent experiments. *p < 0.001.

(C) Hepa1-6 cells were transduced with shRNA lentiviruses against SNAP23, SYN4, SYT7, VAMP7, or a scrambled control and challenged with P. yoelii sporozoites, T. gondii tachyzoites, or T. cruzi trypomastigotes for 90 min. The bar graph displays the infection rate after knockdown of each transcript of interest normalized to scramble shRNA cells, indicated by dashed line (n = 5 for Plasmodium and Trypanosoma; n = 7 for Toxoplasma infection; mean ± SD).

(D) Hepa1-6 cells were incubated with or without the indicated compound for 15 min, washed, and then infected with the indicated parasite for 90 min. The bar graph represents mean ± SD of three independent experiments.

(E) Pearson's correlation coefficients were calculated from the data in (D) for each pairwise combination of infections.