. 2019 Dec 2;20:917. doi: 10.1186/s12864-019-6267-z

Table 4.

List of oligonucleotides

Name	Sequence (5′ → 3′)	Purpose
Cas1_fw	GCGCGCGGCTGGCGCGA	Detection of cas1 gene
Cas1_rev	CGGCGATTGCGTCCGCC	Detection of cas1 gene
Leader_fw	TCACGGGGTCCGCATGAC	PCR amplification of CRISPR array
Terminator_rev	CTCGTCAGCGTCCGGCTG	PCR amplification of CRISPR array
Spacer#19_fw	CGAGCGCATCGATGACGG	PCR amplification of internal region of the CRISPR array
Spacer#21_fw	TCGGGTTTCGGGATGTGC
Spacer#02_rev	CCGGGACGGCGACGAAC
Spacer#18_rev	CGTCGCTGTCGGTGGCAC
IS-1_rev	ACCAGCGCCAGCAGCGG	PCR amplification of internal region of the CRISPR array next to an IS element
IS-2_fw	GCCGACCTGATGATGCA