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. 2019 Oct 23;21:341–358. doi: 10.1016/j.isci.2019.10.038

Figure 6.

Figure 6

Enobosarm Effects on Gene Expression and ER Recruitment Are Reproducible in Different Models

(A) Enobosarm activates AR-target genes and inhibits ER-target genes in HCI-7 xenografts. RNA was extracted from vehicle- or enobosarm-treated HCI-7 tumor tissues (n = 4/group), and expression of the indicated genes was measured by real-time PCR.

(B) Enobosarm inhibits ER recruitment to pS2 promoter in HCI-7 tumors. HCI-7 tumors that were treated with vehicle or enobosarm (n = 3/group) were snap-frozen at the time of collection. Tumors were formalin fixed, homogenized, and ER immunoprecipitated with an ER antibody. Real-time PCR was performed for the pS2 promoter EREs. *p < 0.05.