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. 2019 Nov 8;31:55–66. doi: 10.1016/j.molmet.2019.10.007

Figure 2.

Figure 2

TAZKD mice exhibit normal UCP1 and CL levels in BAT. (A) mRNA levels of PEMT and TAZ during differentiation of preadipocytes to brown adipocytes, n = 2. (B) Knockdown confirmation of PEMT and TAZ mRNA following silencing using the lentivirus system. shRNA against PEMT (shPEMT) or TAZ (shTAZ) administered to preadipocytes via infection with viral media for 48 h. Control cells received a scrambled virus (SC). After infection, preadipocytes were differentiated, n = 2. (C) Protein levels of UCP1, actin, and CS. (D) mRNA levels of UCP1 and other genes involved in adipogenesis, n = 2. (E) TAZ mRNA abundance in BAT, n = 4. (F) Body mass, n = 5–6. (G) Cold-tolerance test, n = 10. (H) Protein levels of UCP1 and CS. (I) Protein abundance of ETS complexes. (J) Mitochondrial phospholipids in BAT measured by TLC. (K) Mitochondrial phospholipids isolated from heart mitochondria. (L) mRNA levels of cardiolipin synthase (CRLS1) and acyl-CoA:lysocardiolipin acyltransferase-1 (ALCAT1), n = 3–5. (M) TAZ mRNA levels in heart, n = 4. Data are expressed as mean ± SEM, *p < 0.05.