Figure 3.

Cell Responses to Optogenetic Stimulus Offered by LEDs

(A) ΔF/F₀ traces from a ChR2-jRCaMP1a co-expressed cell with T_LED = 20 s and I_LED ranging from 0.5 to 1.5 μA.

(B) ΔF/F₀ traces from a ChR2-jRCaMP1a co-expressed cell with T_LED ranging from 10 to 40 s and I_LED = 1.5 μA.

(C) ΔF/F₀ traces from a ChR2-NIR-GECO1 co-expressed cell with T_LED = 20 s and I_LED ranging from 0.5 to 1.5 μA.

(D) Representative ΔF/F₀ traces from a ChR2-NIR-GECO1 co-expressed cell with T_LED ranging from 10 to 40 s and I_LED = 1.5 μA. In (A), (B), (C), and (D), blue windows represent the periods of optogenetic stimulus; solid lines represent the mean values from three consecutive recording periods; shaded areas represent ±1 SD.

(E) ΔF/F₀ signals versus I_LED (left) and T_LED (right) in both co-expressed and control cells. Error bars represent ±1 SD (n = 9 from three independent cells in each group, three recording periods from each cell); **p < 0.01, ***p < 0.001 based on Student's t test.

See also Figures S5 and S6.