Fig. 5.

Western blot analysis of the expression of the exosomal marker proteins. EVs derived from B16F1 (A) and 3T3 Swiss Albino (B) were subjected to SDS-PAGE (1 μg/lane). Western blotting was performed to observe the expression of exosome markers CD9, HSP70, and CD81. Per the product datasheet, the detected molecular weights of CD9, HSP70, and CD81 are approximately 25, 70–78, and 22–26 kDa, respectively. The experiments were performed 3 times independently. The ratios of particle number per protein amount of the EVs collected from B16F1 (C) and 3T3 Swiss Albino (D) were calculated based on the results of Fig. 2, Fig. 3.