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. 2019 Oct 31;18:1009–1022. doi: 10.1016/j.omtn.2019.10.025

Figure 4.

Figure 4

NPAS2 Contributes to HSC Activation

(A) MTS assay for primary HSC proliferation from WT and KO mice as indicated. (B) Cell cycle analysis by flow cytometry in primary HSC proliferation from WT and KO mice as indicated. (C) Col1α1 and α-SMA mRNA expression in primary HSCs from WT and KO mice. (D) Transwell Matrigel invasion assay for cell invasion ability of primary HSCs from WT and KO mice. (E) Flow cytometry analysis of apoptosis by annexin V and propidium iodide (PI) staining in primary HSCs from WT and KO mice. (F) MTS assay for primary HSC proliferation from EV and OE mice as indicated. (G) Cell cycle analysis by flow cytometry in primary HSC proliferation from EV and OE mice as indicated. (H) Col1α1 and α-SMA mRNA expression in primary HSCs from WT and KO mice. (I) Transwell Matrigel invasion assay for cell invasion ability of primary HSCs from EV and OE mice. (J) Flow cytometry analysis of apoptosis by annexin V and PI staining in primary HSCs from EV and OE mice. Data shown are the means ± SEM from three independent experiments. *p < 0.05; **p < 0.01.