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. 2019 Oct 22;60(12):1996–2005. doi: 10.1194/jlr.M094219

Fig. 3.

Fig. 3.

8,9 EET is metabolized by sEH to form 8,9-DHET and COX-2 to form 8,9,11-EHET and 8,9,15-EHET, which are further metabolized to THETs. 8,9-EET (1 µM) was incubated with HAEC cells (300,000) with full media (3 ml) with and without the sEH inhibitor t-TUCB and COX-2 inducer PDBu for 20 h. Levels of lipids (pmol) from the media were analyzed using LC/MS/MS. Values are averages from one to four independent experiments, each having three to four replicates. Error bars are means ± SEs. *P < 0.05 versus the 8,9-DHET formed from PDBu treatment with and without t-TUCB; ‡P < 0.05 versus the 8,9,15-EHET formed from PDBu treatment with and without t-TUCB. Statistical tests were performed using one-way ANOVA with Holm-Sidak pairwise analysis. The raw data used for this figure are reported in supplemental Table S2.