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. 2019 Oct 22;60(12):1996–2005. doi: 10.1194/jlr.M094219

Fig. 5.

Fig. 5.

8,9,11-EHET enhances HAEC tube formation. A: HAECs in basal media were seeded onto a 15-well µ-angiogenesis plate with growth factor-reduced Matrigel. Cells were treated with vehicle, VEGF (2 ng/ml), or various concentrations of 8,9,11-EHET or 8,9,15-EHET for 24 h. Tube formation was observed using a fluorescence microscope after staining with Calcein. Tube length was measured using Fiji. B: Representative images of HAEC tube formation after a 24 h incubation with vehicle and the 8,9,11-EHET treatment (0.1 µM). Values are means ± SEs (n = 3). *P < 0.05 versus the control. Statistical tests were performed using one-way ANOVA with Holm-Sidak comparison to the control analysis. The raw data used for this figure are reported in supplemental Table S4.