TABLE 1.

PDBu induces HAEC tube formation more than 8,9-EET and 8,9,11-EHET treatments

Treatment	Tube Length vs. Control (%)	Sample size (n)
Vehicle	100 ± 11	12
VEGF	220 ± 34*	5
8,9-EET	209 ± 31	4
8,9,11-EHET	242 ± 23*	3
8,9,15-EHET	85 ± 28	3
t-TUCB	194 ± 27	4
PDBu	681 ± 30*	4

HAECs in basal media (in the absence of serum and VEGF) were seeded onto a 15-well µ-angiogenesis plates with growth factor-reduced Matrigel. Cells were then treated with vehicle (ethanol) or separately incubated with 8,9-EET (0.1 µM), 8,9,11-EHET (0.1 µM), or PDBu (1 µM). Tube length was measured manually using Fiji. The analysis was blinded toward treatments to avoid bias. Values are means ± SEs (n = 3–12). *P < 0.05 versus the control. Statistical tests were performed using one-way ANOVA with Holm-Sidak comparison to the control analysis.