Fig. 6.

Effects of modulation ω-6:ω-3 PUFA ratio on EtOH- and LPS-mediated alterations in fecal BA profiles and related metabolic pathways. A: Fecal primary and secondary BA concentrations (n = 3–7). Expression of genes related to BA detoxification in the ileum (RNA-seq) (B) and in the liver (C) as determined by qRT-PCR in WT and fat-1 mice. Expression of genes involved in BA synthesis (D), transport (E), and reuptake (F) in the liver. G, H: Expression of Nr1h4/Fxr, Nr1i2/Pxr, and Nr1i3/Car in the ileum and liver, respectively (n = 3–5). CA, cholic acid, DCA, deoxycholic acid.