Fig. 1.

CXCL12 reduced ABCA1 expression for the inhibition of cholesterol efflux in THP-1-derived macrophages. The effect of CXCL12 on ABCA1 expression and cholesterol efflux. THP-1-derived macrophages were incubated with 80 ng/ml CXCL12. A: Analysis of mRNA and protein levels of ABCA1 using quantitative RT-PCR and Western blot, respectively. B: Analysis of ABCA1 by immunofluorescent staining. Scale bar = 20 μm. C: Analysis of cholesterol efflux to apoA-I and HDL using liquid scintillation counting assays. All data are shown as means ± SDs from three independent experiments, each experiment performed in triplicate. *P < 0.05 versus the control group.