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. 2019 Oct 29;60(12):2020–2033. doi: 10.1194/jlr.RA119000100

Fig. 2.

Fig. 2.

CXCL12 downregulated TCF21 expression and inhibited the binding of TCF21 to the ABCA1 promoter. The role of TCF21 in CXCL12-induced reduction ABCA1 expression. A, B: HEK293T cells were transfected with TCF21 and ABCA1 promoter 3000 or incubated with 80 ng/ml CXCL12 and transfected with ABCA1 promoter 3000. ABCA1 promoter activity was analyzed using the luciferase reporter gene assay. C: THP-1-derived macrophages were transfected with ABCA1 promoter 3000 and TCF21 and then incubated with or without 80 ng/ml CXCL12. Analysis of the action of CXCL12 on the binding of TCF21 to the ABCA1 promoter region using the chromatin immunoprecipitation assay. D, E: THP-1-derived macrophages were transfected with or without TCF21 and then incubated with 80 ng/ml CXCL12. mRNA and protein levels of TCF21 (D) or ABCA1 (E) were measured using quantitative RT-PCR and Western blot, respectively. All data are shown as means ± SDs from three independent experiments, each performed in triplicate. *P < 0.05 versus the control group or IgG group. *P < 0.05 versus the ABCA1 promoter 3000 group or TCF21 of the control group or TCF21 group.