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. 2019 Oct 29;60(12):2020–2033. doi: 10.1194/jlr.RA119000100

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Copyright © 2019 Gao et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 5. — Binding of CXCL12 to CXCR4 was required for the reduction in ABCA1. The role of CXCR4 in CXCL12-induced reduction in ABCA1 expression. A: THP-1-derived macrophages were incubated with 80 ng/ml CXCL12. The interaction between CXCL12 and CXCR4 was examined using coimmunoprecipitation. B, C: Detection of the silent efficiency of CXCR4 siRNA using Western blot and an immunofluorescence assay. Scale bar = 20 μm. D, E: THP-1-derived macrophages were incubated with 80 ng/ml CXCL12 and/or treated with CXCR4 siRNA or LY2510924. Analysis of the levels of phosphorylated GSKβ (D) and protein levels of β-catenin (F) and levels of phosphorylated β-catenin^Y654 and β-catenin^T120 (E) using Western blot. G, H: The measurement of mRNA and protein levels of TCF21 (F) and ABCA1 (G) using quantitative RT-PCR and Western blot, respectively. All data are shown as means ± SDs from three independent experiments, each performed in triplicate. *P < 0.05 versus the control group.

Fig. 5. — Binding of CXCL12 to CXCR4 was required for the reduction in ABCA1. The role of CXCR4 in CXCL12-induced reduction in ABCA1 expression. A: THP-1-derived macrophages were incubated with 80 ng/ml CXCL12. The interaction between CXCL12 and CXCR4 was examined using coimmunoprecipitation. B, C: Detection of the silent efficiency of CXCR4 siRNA using Western blot and an immunofluorescence assay. Scale bar = 20 μm. D, E: THP-1-derived macrophages were incubated with 80 ng/ml CXCL12 and/or treated with CXCR4 siRNA or LY2510924. Analysis of the levels of phosphorylated GSKβ (D) and protein levels of β-catenin (F) and levels of phosphorylated β-catenin^Y654 and β-catenin^T120 (E) using Western blot. G, H: The measurement of mRNA and protein levels of TCF21 (F) and ABCA1 (G) using quantitative RT-PCR and Western blot, respectively. All data are shown as means ± SDs from three independent experiments, each performed in triplicate. *P < 0.05 versus the control group.