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. 2019 Oct 11;60(12):1983–1995. doi: 10.1194/jlr.M093252

Fig. 3.

Fig. 3.

Deficiency of AGT in hepatocytes had minor effects on hepatic lipid utilization. A: Liver mRNA abundances of major genes involved in lipolysis, thermogenesis, and fatty acid oxidation were not different between hepAGT−/− and hepAGT+/+ mice fed a Western diet (N = 5–9 for each group). Comparison between genotypes by Student’s t-test. B: The fatty acid oxidation assays of liver mitochondria isolated from hepAGT+/+ mice or hepAGT−/− mice fed a Western diet, respectively (N = 3 for each group). C: The capacity of liver mitochondria fatty acid oxidation interpreted by oxygen consumption rate was similar between hepAGT−/− mice and hepAGT+/+ mice fed a Western diet using a high-resolution respirometry (N = 3 for each group). Comparison between genotypes by Student’s t-test. D: The contents of plasma triglycerides were similar irrespective of genotype and diet (N = 6–9 for each group). Comparison among groups by ANOVA on Ranks. E: The abundance of autophagy-related proteins in liver tissues from hepAGT−/− and hepAGT+/+ mice fed a Western diet (N = 5 for each group). F: The protein abundance of liver LC3 was detected in hepAGT+/+ and hepAGT−/− mice fed a Western diet receiving either Baf A1 or vehicle, respectively (N = 3 for each group). G: Autophagic flux index calculated as the ratio of LC3-II protein abundance post/pre-Baf A1 injection was similar between hepAGT+/+ and hepAGT−/− mice fed a Western diet (N = 3 for each group). Comparison between genotypes by Student’s t-test. ND, normal laboratory diet; WD, Western diet; FAO, fatty acid oxidation; Mal, malate; Pal, palmitoylcarnitine; Oct, octanoylcarnitine; CytC, cytochrome C; ADP, adenosine diphosphate; Rot, rotenone; ATG7, autophagy related 7; ATG12, autophagy related 12; P62, also known as SQSTM1; Baf A1, bafilomycin A1; N.S., not significant.