Supplementary Table 1.

Formulation of Culture Media for Human Colonic Epithelial Cells

	SM	EM	DM	DM-VIP
WRN-conditioned medium	50 vol%	50 vol%
Advanced DMEM/F12	50 vol%	50 vol%	100 vol%	100 vol%
GlutaMax	1×	1×	1×	1×
HEPES	10 mM	10 mM	10 mM	10 mM
Murine EGF	50 ng/mL	50 ng/mL	50 ng/mL	50 ng/mL
N-acetyl cysteine	1.25 mM	1.25 mM	1.25 mM	1.25 mM
Primocin	50 μg/mL	50 μg/mL	50 μg/mL	50 μg/mL
B27	1×	1×
Gastrin	10 mM	10 mM
A83-01	500 nM		500 nM	500 nM
SB202190	3 μM	3 μM
Y-27632	10 μMa	10 μMa
Nicotinamide		10 mM
PGE2		10 nM
VIP				330 ng/mL

Advanced DMEM/F-12, Dulbecco's Modified Eagle Medium/Ham's F-12; DM, differentiation medium; EGF, epidermal growth factor; EM, expansion medium; WRN, Wnt-3A, R-spondin 3, Noggin; PGE2, prostaglandin E2; SM, stem medium; VIP, vasoactive intestinal peptide.

^aUsed in the first 48 hours after cell plating to prevent dissociation-induced cell apoptosis.