Figure 3.

Loss of Mist1^–/–in small intestine does not alter secretory allocation. (A) Staining for sucrase-isomaltase (enterocytes), alcian blue (goblet cells), and lysozyme (Paneth cells). Scale bars equal 100 μm, 50 μm, and 50 μm, respectively. Black arrows point out alcian blue⁺ Paneth cells. (B) Quantification of chromogranin (Cg) A⁺ cells in crypt and villus compartments. n = 3 mice per group. Quantification of alcian blue⁺ cells in crypt and villus compartments. n = 3 mice per group. **P <.01. Quantification of lysozyme⁺ cells per crypt. n = 3 mice per group. (C) mRNA expression of transcription factors associated with secretory cell lineage allocation in isolated jejunal crypts from Mist1^+/+ and Mist1^–/– mice. n = 3–6 mice per group.