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. 2019 Jul 19;8(4):549–560. doi: 10.1016/j.jcmgh.2019.07.003

Figure 4.

Figure 4

Mist1 expression prevents Paneth cells from becoming intermediate cells. (A) Immunofluorescence staining of jejunal tissue from Mist1+/+ and Mist1–/– mice for muc2 (green) and lysozyme (red). Dual positive cells (yellow) are intermediate cells. Scale bar equals 50 μm. (B) Representative PTAB staining of jejunal tissue from Mist1+/+ and Mist1–/– mice. Phloxine tartrizine stains protein-dense areas, Paneth cell–like granules brown, and alcian blue stains mucins blue. Paneth cell granules from Mist1+/+ Paneth cells stain brown (white arrows), and granules from Mist1–/– Paneth cells, which contain mucins, stain purple, suggesting an intermediate cell phenotype (black arrows). Scale bar equals 10 μm. (C) Quantification of PTAB staining of jejunal tissue from Mist1+/+ and Mist1–/– mice from (B) showing alcian blue+ (AB), phloxine/tartrizine+ (PT), dual positive (PTAB), and total stained cells (PT + PTAB) per crypt. n = 3 mice per group. *P <.05. **P <.01. ****P <.0001.