Skip to main content
. 2019 Jul 19;8(4):549–560. doi: 10.1016/j.jcmgh.2019.07.003

Figure 6.

Figure 6

Intermediate cell phenotype of Mist1–/–mice is not due to impaired Notch signaling. (A) Mist1+/+ and Mist1–/– mice were treated with DBZ to inhibit Notch signaling, and jejunal tissues were stained with alcian blue. Scale bar equals 125 μm. (B) Jejunal tissues from (A) were stained with PTAB to evaluate Paneth cells (yellow arrows) and intermediate cells (black arrows). Paneth cell granules from Mist1+/+ Paneth cells stain brown, and granules from Mist1–/– Paneth cells that contain mucins stain purple, suggesting an intermediate cell phenotype. Scale bar equals 50 μm. (C) Quantification of Paneth cell and intermediate cell numbers. n = 4 mice per group. ****P <.0001. **P <.01.