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. 2019 Nov 25;10:1449. doi: 10.3389/fpls.2019.01449

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Copyright © 2019 Jain, Dashner and Connolly

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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mit mutants exhibit altered Fe homeostasis under Fe deficiency. (A) Root ferric reductase activity assay on seedlings grown in Fe-sufficient or Fe-deficient media. The activity was normalized to root fresh weight. Values shown are an average of 10 biological replicates. Error bars in all the graphs indicate standard deviation. Significance was assessed using the student’s t-test. The asterisk represents a p < value 0.05 as compared to Col-0. Seedlings were grown on standard B5 media for 2 weeks and then transferred to Fe-sufficient (50 µM Fe(III)-EDTA) or Fe-deficient (300 µM ferrozine media) for 3 days. (B) Immunoblot of IRT1 protein levels in the roots of mit mutants grown on standard B5 media for 2 weeks and then transferred to Fe-deficient conditions for 3 days. (C) Immunoblot of ferritin levels in shoots of 2.5-week-old mit seedlings grown in Fe-sufficient media. 25 µg of protein lysate was loaded per lane for the immunoblots.