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. 2019 Nov 25;10:1449. doi: 10.3389/fpls.2019.01449

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Copyright © 2019 Jain, Dashner and Connolly

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Arabidopsis MIT1 and MIT2 complement the defective growth phenotype of mrs3mrs4 on Fe-deficient media. mrs3mrs4 was transformed with MIT1, MIT2, MRS3 (positive control) and the empty vector (pRS426-ADH; negative control). Three serial dilutions corresponding to OD₆₀₀ of 1, 0.1 and 0.01 of the transformed yeast were assayed for growth complementation on SD-uracil plates under Fe-sufficient (0.1mM FeSO₄) or Fe-deficient (0.1mM BPS) conditions.