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. 2019 Nov 25;10:1449. doi: 10.3389/fpls.2019.01449

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Copyright © 2019 Jain, Dashner and Connolly

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantitative RT-PCR expression analysis of MIT1 and MIT2 in shoots and roots of WT seedlings grown in standard B5 media for 2 weeks and then transferred to Fe-sufficient or Fe-deficient media for 3 days. The mRNA levels for both MIT1 and MIT2 from +Fe shoot was set at 1 and the relative abundance of the two transcripts were determined in the other tissues. Actin was used as a reference gene to normalize mRNA values. Values represent the mean of 3 biological replicates, and error bars indicate standard deviation. Significance (p < 0.05) was assessed using the Student’s t-test; significant differences in the transcript abundance between +Fe and −Fe shoots is represented by an asterisk.