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. 2019 Nov 25;10:1449. doi: 10.3389/fpls.2019.01449

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Copyright © 2019 Jain, Dashner and Connolly

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Genetic analysis of mit1 and mit2 mutants. (A) Gene structure of the mit1 and mit2 T-DNA insertion mutants. The insertion in mit1 was identified in its first exon, 246 bases after the translation start site, the insertion in mit2 is located in the intron, 1592 bases after the translation start site. (B) Relative quantification (RQ) of MIT1 and MIT2 transcript levels by quantitative RT-PCR in mit1 and mit2, respectively. (C) Relative quantification (RQ) of MIT1 and MIT2 in Col-gl-1 and amiRmit1mit2 lines. Actin was used a control. The values represent the mean of two to three biological replicates, and error bars indicate standard deviation. The RNA was harvested from 2.5-week-old seedlings grown in Fe-sufficient media.