Table 1.

Examples of lysis buffers for isolating DNA sensors.

DNA sensor	Tag	Cell type	Lysis buffer	Reference
TLR9	Myc-tagged	HEK293T	50mM Tris–HCl, pH 8.0, 150mM NaCl, 5mM EDTA, 1% digitonin, and 1 × PIC	Lee, Kang, and Kim (2016)
IFI16	(A,B,D) Endogenous (C,E)GFP-tagged	(A)CEM-T (B,C,D,E)HFF (C)THP-1 (C)HEK293	20mMK-Hepes, pH 7.4, 0.11M KOAc, 2mM MgCl2, 0.1% Tween-20, 1 μM ZnCl2, 1 μM CaCl2, 0.6% Triton X-100, 200mM NaCl, (Pierce), 1 × PIC, 1 × PHIC, 100U/mL Benzonase.	A. Li, et al. (2012)
				B.Diner, Lum, et al. (2015)
				C.Diner, Li, et al. (2015)
				D. Orzalli et al. (2015)
				E. Diner, et al. (2016)
IFIX	GFP-tagged	HEK293	20 mM K-Hepes, pH 7.4, 0.11M KOAc, 2mM MgCl2, 0.1% Tween-20, 1 μM ZnCl2, 1 μM CaCl2, 0.6% Triton X-100, 200mM NaCl, (Pierce), 1 × PIC, 1 × PHIC, 100U/mL Benzonase.
				A.Diner, Li, et al. (2015)
				B.Crow and Cristea (2017)
AIM2	GFP-tagged	HEK293	20 mM K-Hepes, pH 7.4, 0.11M KOAc, 2mM MgCl2, 0.1% Tween-20, 1 μM ZnCl2, 1 μM CaCl2, 0.6% Triton X-100, 200mM NaCl, (Pierce), 1 × PIC, 1 × PHIC, 100U/mL Benzonase.	Diner, Li, et al. (2015)
cGAS	(A,B)FLAG- tagged (C) HA-tagged (D) GFP-tagged	(A,B,C)HEK- 293 T (D)HFF	(A) 20 mM K-Hepes, pH 7.4, 0.11M KOAc, 2mM MgCl2, 0.1% Tween-20, 1 μM ZnCl2, 1 μM CaCl2, 0.6% Triton X-100, 200mM NaCl, (Pierce), 1 × PIC, 1 × PHIC, 100U/mL Benzonase. (B) 1% NP-40 buffer supplemented with a complete protease inhibitor cocktail (Roche). (C) 50mM Tris, pH 7.4, 150mM NaCl, 1% Triton X-100, 1 mM EDTA (pH 8.0), 1 mM PMSF, 1 mM Na3VO4, 1 mM of NaF. (D) Cytoplasmic fraction: (20mM HEPES, pH 7.4, 10mM KCl, 2mM MgCl2, 1 mM DTT, 0.5% NP-40, 1 × PIC and 1 × PhIC), 100U/mL Benzonase. Nuclear fraction: cytoplasmic lysis buffer supplemented with 200 mM NaCl, 1% Triton X-100, 0.11M KOAc, 1% Tween-20, 100U/mL Benzonase.
				A.Diner, et al. (2016)
				B. Seo et al. (2018)
				C.Liu et al. (2018)
				D.Lum, et al. (2018)