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. Author manuscript; available in PMC: 2019 Dec 3.
Published in final edited form as: Dev Dyn. 2013 Oct 7;242(11):1320–1331. doi: 10.1002/dvdy.24021

Figure 2. NRSS Stages 0 through 2.

Figure 2

(A) Stage 0, Open Wound. The wound site may be visibly expanding and contracting, and is seen here in a contracted position. (B) Stage 1, Closed Wound. The wound has closed, although the mesenterial insertions may not meet in an organized fashion at the new oral pole. (C) Stage 2, Radial Arches. The puffed arches, appearing constrained at the mesenterial insertions, give the oral pole the appearance of a stalk-less pumpkin. (D) High magnification view of tissue blebs (dotted orange line). The bleb is nearly spherical in shape and is a single cell layer thick. (E) High magnification view of tentacle buds (dotted red line). Tenacle buds are wider at the base than tip, may appear mound-shaped or pointed, and are two cell layers thick. (A) and (B) are oriented with the oral pole facing out from the page. (C–E) are oriented with the oral pole to the right. Asterisks indicate site of amputation/oral pole. Scale bars are 0.5mm (A–C) and 0.1mm (D,E).