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Fig. 5. Interface pH′ (–log[H+]) of DOPC/DOPE/TOCL (2 : 1 : 1) LUVs (total lipid, 0.04 mM) plotted against cyt c concentration at 25 °C in 10 mM HEPES at various pHs: blue, pH 6.5; red, pH 7.0. The plots of interface pH′ in the absence of cyt c at pH 6.5 and 7.0 are depicted in red and blue broken lines, respectively, for comparison.