Table 2. Kinetic parameters for ABTS oxidation by the peroxidase reaction of cyt c in the presence of DOPC/DOPE/TOCL LUVs containing TOCL or TMCL (TOCL or TMCL = 0.25 mM) and DOPG LUVs (0.5 mM) at pH 6.8, and those in the absence of LUVs at bulk pH corresponding to the interface pH′. Measurements were performed at 25 °C.

LUV	Bulk pH	Interface pH′	k cat (s–1)	K m (μM)
None	6.80		2.0 ± 0.1	10.0 ± 0.4
DOPG	6.80	5.30	4.7 ± 0.2	11.8 ± 0.5
None	5.30		5.5 ± 0.2	12.3 ± 0.5
10% TOCL a	6.80	4.32	11.3 ± 0.4	12.7 ± 0.5
None	4.32	—	11.2 ± 0.4	14.0 ± 0.6
25% TOCL a	6.80	3.90	15.5 ± 0.5	13.5 ± 0.5
None	3.90	—	14.4 ± 0.5	13.2 ± 0.6
50% TOCL a	6.80	3.61	31.9 ± 1.0	14.0 ± 0.5
None	3.61		15.9 ± 0.5	14.1 ± 0.5
10% TMCL b	6.80	4.25	10.3 ± 0.4	12.1 ± 0.5
None	4.25		11.1 ± 0.4	11.9 ± 0.5
25% TMCL b	6.80	3.95	13.7 ± 0.5	13.5 ± 0.5
None	3.95		12.9 ± 0.5	13.3 ± 0.5
50% TMCL b	6.80	3.55	15.7 ± 0.5	13.9 ± 0.6
None	3.65		15.5 ± 0.5	13.7 ± 0.5

^aDOPC/DOPE/TOCL LUVs (DOPC : DOPE = 2 : 1, TOCL = 10, 25, and 50% of total lipids).

^bDOPC/DOPE/TMCL LUVs (DOPC : DOPE = 2 : 1, TMCL = 10, 25, and 50% of total lipids).