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. 2019 Nov 26;10:2779. doi: 10.3389/fimmu.2019.02779

Figure 4.

Figure 4

Monocyte-derived APCs expressed gene signatures for both dendritic cells and macrophages. (A–F) The gene expression level of Itgax (A), H2-Ab1 (B), H2-Aa (C), Cd68 (D), Mertk (E), and Cd74 (F) in monocytes isolated from the bone marrow (BM-Mono) or spinal cords (SC-Mono), and the monocyte-derived APCs isolated from the spinal cords (SC-APC) at days 14–15 following EAE induction. Shown are TPM values from RNA-Seq analysis. (G–J) Spinal cord cells were isolated at days 14–15 (G) or day 7 (H) following EAE induction. The expression of surface markers for dendritic cells (CD11c, MHC II) and macrophages (MerTK, CD24) in the CD45hi CD11bhi CD64+ Ly6G− cells or the CD45hi CD11bhi CD64− Ly6G− cells was determined. Shown are representative plots from three independent experiments, with a total of 10–11 animals in each time point. (I) Percentage of cells expressing MerTK and CD24 within the CD11c/MHCII subpopulations from individual animals is shown. (J) Percentage of cells expressing CD11c and MHCII within the CD64hi or CD64lo/− populations from individual animals is shown. *P < 0.05; **P < 0.01; ***P < 0.001. NS, not statistically different.