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. 2019 Nov 26;10:2779. doi: 10.3389/fimmu.2019.02779

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Copyright © 2019 Monaghan, Zheng, Hu and Wan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The expression of Ccl17, Ccl22, and Ccr7 distinguishes monocytes from monocyte-derived APCs. (A–D) Heatmaps showing the differential expression of genes that encode cytokines (A), cytokine receptors (B), chemokines (C), and chemokine receptors (D) from bone marrow monocytes relative to the spinal cord monocytes, and/or spinal cord monocytes relative to the spinal cord monocyte-derived APCs. Colors on the heatmap represent log₂ values of TPM. (E–G) Gene expression level of Ccl17 (E), Ccl22 (F), Ccr7 (G) in monocytes isolated from the bone marrow (BM-Mono) or spinal cords (SC-Mono), and the monocyte-derived APCs isolated from the spinal cords (SC-APC) at days 14–15 following EAE induction. Shown are TPM values from RNA-Seq analysis.