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Immunohistochemistry of VTA neurons in VGAT-Venus rats. Double immunofluorescenct labeling of VTA neurons shows VGAT Venus-positive cells (green, left), NeuN positive cells (red, center) and an overlay showing the overlap (right). As not all neurons are VGAT+, we provide evidence that we were able to selectively identify and record from GABAergic neurons. Calibration bar on the right panel denotes 150 µm.
