Skip to main content
. Author manuscript; available in PMC: 2020 Feb 8.
Published in final edited form as: ACS Infect Dis. 2018 Dec 13;5(2):208–217. doi: 10.1021/acsinfecdis.8b00205

Figure-4: Effect of antisense fabK on sporulation.

Figure-4:

(Panel A, B & C) Sporulation was analyzed over 5 days as the total number of spores/total population (spore forming and vegetative cells). (A) Colonies obtained from BHI agar plate with 0.1% (w/v) taurocholate were inoculated into BHI medium and grown until OD600 ≈ 0.3, at which antisense RNA expression was induced by addition of 0, 0.016 or 0.128 μg/mL of ATc or 113 μM acridine orange (AO). (B) Comparison of inhibition of sporulation by cerulenin (CRN; MIC=16 μM) and vancomycin (VAN; MIC=0.25 μM). DMSO and AO were negative and positive controls.