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. Author manuscript; available in PMC: 2020 Nov 27.
Published in final edited form as: Cell. 2019 Nov 27;179(6):1276–1288.e14. doi: 10.1016/j.cell.2019.10.034

Figure 3. GPR146 Promotes ERK1/2 Activities in Hepatocytes Upon Feeding.

Figure 3.

(A) GSEA enrichment plot of MAPK_Signaling gene set (KEGG pathway database) in liver of Gpr146+/+ mice and Gpr146−/− littermates (n=3 mice per group) upon 6-hour refeeding after a 16-hour fast.

(B and C) Western blot (B) and relative quantification (C) of phosphorylated ERK1/2 (pERK1/2) and total ERK1/2 in liver of 6-hour refed Gpr146+/+ mice and Gpr146−/− littermates (n=5 mice per group, by Student’s t-test).

(D and E) Western blot (D) and relative quantification (E) of phosphorylated ERK1/2 (pERK1/2) and total ERK1/2 in liver of 16-hour fasted Gpr146+/+ mice and Gpr146−/− littermates (n=7 mice per group, by Student’s t-test).

(F) Western blot of phosphorylated ERK1/2 (pERK1/2) and total ERK1/2 in liver of 16-hour fasted male mice (C57BL/6J) or 6-hour refed littermates upon chow diet feeding (n=4 mice per group).

(G and H) Western blot (G) and relative quantification (H) showing upregulated pERK1/2 levels with recombinant expression of human GPR146 in HepG2 cells upon stimulation with serum for the time indicated (n=2 replicates per genotype per experiment, representative of 3 independent experiments, by Student’s t-test).

* p< 0.05, ** p< 0.01; bars in C indicate mean± s.e.m., bars in E and H indicate mean ± s.d..