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. Author manuscript; available in PMC: 2020 Nov 27.
Published in final edited form as: Cell. 2019 Nov 27;179(6):1276–1288.e14. doi: 10.1016/j.cell.2019.10.034

Figure 5. GPR146 Regulates ERK/SREBP2 Axis in Mice upon Short Period of Fasting.

Figure 5.

(A) Schedule of fasting and refeeding treatment in vivo.

(B and C) Western blot (B) and relative quantification (C) of phosphorylated ERK1/2 (pERK1/2) and total ERK1/2 in livers of mice upon different fasting refeeding regimens (n=3 mice per group, by one-way ANOVA).

(D and E) Western blot (D) and relative quantification (E) of SREBP2 precursor (P-SREBP2), mature SREBP2 (M-SREBP2), pERK1/2 and total ERK1/2 in livers of 6-hour fasted Gpr146+/+ mice and Gpr146−/− littermates (n=5 mice per group, by Student’s t-test).

(F) Quantitative polymerase chain reaction (qPCR) expression analysis of cholesterol biosynthetic genes in liver of 6-hour fasted Gpr146+/+ mice and Gpr146−/− littermates (n=5 mice per group, by Student’s t-test).

* p< 0.05, ** p< 0.01; bars in C and E indicate mean± s.d., bars in F indicate mean± s.e.m..