Key Resource Table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-HSP90 | Santa Cruz Biotechnology | Cat# sc-13119; RRID:AB_ 675659 |
| Mouse monoclonal anti-APOB | Santa Cruz Biotechnology | Cat# sc-393636 |
| Rabbit monoclonal anti-p44/42 MAPK (Erk1/2) | Cell Signaling Technology | Cat# 4695; RRID:AB_390779 |
| Rabbit monoclonal anti-p38 MAPK | Cell Signaling Technology | Cat# 8690; RRID:AB_ 10999090 |
| Rabbit polyclonal anti-SAPK/JNK | Cell Signaling Technology | Cat# 9252; RRID:AB_ 2250373 |
| Rabbit monoclonal anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) | Cell Signaling Technology | Cat# 4370; RRID:AB_ 2315112 |
| Rabbit polyclonal anti-phospho-p38 MAPK (Thr180/Tyr182) | Cell Signaling Technology | Cat# 9211; RRID:AB_ 331641 |
| Rabbit monoclonal anti-phospho-SAPK/JNK (Thr183/Tyr185) | Cell Signaling Technology | Cat# 9251; RRID:AB_ 331659 |
| Rabbit monoclonal anti-HMGCS1 | Cell Signaling Technology | Cat# 42201; RRID:AB_2799216 |
| Rabbit polyclonal anti-SREBP2 | Abcam | Cat# Ab30682; RRID:AB_ 779079 |
| Rabbit polyclonal anti-HMGCR | Thermo Fisher Scientific | Cat# PA5–37367; RRID:AB_2554032 |
| Rabbit polyclonal anti-LDLR | Sigma-Aldrich | Cat# HPA013159, RRID:AB_1852775 |
| Mouse monoclonal anti-β-actin | Sigma-Aldrich | Cat# A5316; RRID:AB_ 476743 |
| Mouse monoclonal anti-FLAG | Sigma-Aldrich | Cat# F3165; RRID:AB_ 259529 |
| Bacterial and Virus Strains | ||
| AAV8-GFP-U6-scrmb-shRNA | Vector Biolabs | N/A |
| AAV8-GFP-U6-rm-Gpr146-shRNA | Vector Biolabs | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| PD0325901 | Selleckchem | S1036 |
| Poloxamer-407 | Sigma-Aldrich | 16758 |
| Low Density Lipoprotein from Human Plasma, Dil Complex | Thermo Fisher Scientific | L3482 |
| Critical Commercial Assays | ||
| Infinity Triglycerides Reagent | Thermo Fisher Scientific | TR22421 |
| Infinity Cholesterol Reagent | Thermo Fisher Scientific | TR13421 |
| HDL and LDL/VLDL Quantitation Kit | Sigma-Aldrich | MAK045–1KT |
| Human ApoB ELISAPRO kit | Mabtech | 3715–1HP-2 |
| Deposited Data | ||
| Microarray Raw data | This paper | GEO: GSE139082 |
| Experimental Models: Cell Lines | ||
| Human: HEK293T | ATCC | ATCC Cat# CRL-3216, RRID:CVCL_0063 |
| Human: Huh7 | JCRB Cell Bank | JCRB Cat# JCRB0403, RRID:CVCL_0336 |
| Human: HepG2 | ATCC | ATCC Cat# HB-8065, RRID:CVCL_0027 |
| Mouse: AML12 | ATCC | ATCC Cat# CRL-2254, RRID:CVCL_0140 |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57BL/6J | The Jackson Laboratory | RRID:IMSR_JAX:000664 |
| Mouse: B6.Cg-Speer6-ps1Tg(Alb-cre)21Mgn/J | The Jackson Laboratory | RRID:IMSR_JAX:003574 |
| Mouse: B6; FVB-Tg(Adipoq-cre)1Evdr/J | The Jackson Laboratory | RRID:IMSR_JAX:010803 |
| Mouse: B6. 129S7-Ldlrtm1Her/J | The Jackson Laboratory | RRID:IMSR_JAX:002207 |
| Mouse: Gpr146−/− | This paper | N/A |
| Mouse: Gpr146fl/fl | This paper | N/A |
| Mouse: Gpr146-LKO | This paper | N/A |
| Mouse: Gpr146-FKO | This paper | N/A |
| Mouse: Gpr146−/−/ Ldlr−/− | This paper | N/A |
| Oligonucleotides | ||
| Gpr146 sgRNA1 targeting sequence: GCTACCTTTTGGCACGGGTT | This paper | N/A |
| Gpr146 sgRNA2 targeting sequence: GTGGCTAAGGGATTATATGG | This paper | N/A |
| loxp ssDNA template1: TGACCCACCAGAGAGGCTTTTGTGTGCCTTCCTGTGCCACTACTCCACCAGGAGCAGAGCTGCTGCTACCTTTTGGCACGATAACTTCGTATAATGTATGCTATACGAAGTTATGCTAGCGGTTAGGGGACTCTTTGGGGACAGAACCCACAGAACCTCAAACCACTTGGCAGTGCAAGGCTGCCTGCCTCCTTGCCCCC | This paper | N/A |
| loxp ssDNA template2: GAGTTTGAATCTAAGCCCAGATGCACAGACCTTGTTTCAAGTCAAAGTCTTCAGGGCTGCAAAAGTGGCTAAGGGATTATGAGCTCATAACTTCGTATAGCATACATTATACGAAGTTATATGGTGGAGCACAGCTATAATCCCAGCACTTACTAGGCAGGGGCAGGTGGATCTCTGTGAGTTTGAGGCCATCTTAGTCG | This paper | N/A |
| Gpr146 AAV8-shRNA targeting sequence: GCATTATCTGGGCATCCTACA | Vector Biolabs | N/A |
| see Table S2 for additional oligonucleotides | This paper | N/A |
| Recombinant DNA | ||
| Human GPR146 cDNA | Mammalian Gene Collection | Clone ID: 4563636 |
| Plasmid: lentiCas9-Blast | Sanjana et al., 2014 | RRID:Addgene_52962 |
| Plasmid: lentiGuide-Puro | Sanjana et al., 2014 | RRID:Addgene_52963 |
| Plasmid: FU-tetO-Gateway | Addis et al., 2011 | RRID:Addgene_43914 |
| Plasmid: FU-tetO-GPR146 | This paper | N/A |
| Plasmid: FU-tetO-GPR146-FLAG | This paper | N/A |
| Plasmid: FU-tetO-eGFP-FLAG | This paper | N/A |
| Software and Algorithms | ||
| ImageJ | NIH | https://imagej.nih.gov/ij/ |
| GraphPad Prism5 | GraphPad Software | https://www.graphpad.com/ |