Figure 2.
Effect of Lamtor1 deficiency on insulin production. (A) Demonstration of successful Lamtor1 knockout in βLamtor1-KO mice. Western blot showing the depletion of Lamtor1 in islet lysates. (B) IPGTT was conducted on 8-week-old mice after 12 h of fasting, and blood glucose levels were measured. Data are shown as mean ± SD. *P<0.05, n=6. (C) Expression of Pdx1, Ins2, Glut2, and Gck was detected by real-time fluorescence quantitative-PCR, n=6. (D) Insulin content from the islets of fed and fasted βLamtor1-KO mice and Lamtor1F/F littermate controls. Insulin content is presented relative to that of the pancreas. n=6. IPGTT, intraperitoneal glucose tolerance test; Lamtor1, late endosomal/lysosomal adaptor MAPK and mTOR activator 1; KO, knockout; NS, no significance.