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. 2019 Nov 21;45(1):23–32. doi: 10.3892/ijmm.2019.4409

Figure 3.

Figure 3

Loss of Lamtor1 enhances glucose-stimulated insulin secretion. Result of hyperglycemic clamp in Lamtor1F/F and βLamtor1-KO mice. (A) Blood glucose levels. A total of 20% glucose was injected through the jugular vein catheter to maintain blood glucose levels at the 16.0-18.0 mM for 90 min. (B) Glucose infusion rate. Glucose infusion rates calculated by calculating the average amount of glucose (mg/kg/min) in 90 min. (C) Detection of insulin secretion levels. 20-30 µl blood samples were collected from the tail vein at 0, 1, 5, 20, 50, 70 and 90 min after glucose infusion to detect insulin levels. Data are shown as mean ± SD. *P<0.05, **P<0.01, n=6. (D) Dynamic insulin secretion assay. The levels of insulin secretion were measured after adding 2.8 mM glucose and 16.7 mM glucose respectively during perfusion of isolated islets. The addition of 30 mM KCl led to membrane depolarization, which increased insulin secretion. The insulin measured for each sample was normalized to total DNA. n=6.