MgTX decreases the expression of HF-associated proteins in RAW264.7 cells. To confirm the results of HF-associated proteins from immunohisto-chemistry in RAW264.7 cells, (A) western blot analysis was performed to examine the expression of MMP12, MM13, POSTN, CCL2, Kv1.3 and δ-catenin, which were downregulated by MgTX in the M1 phenotype groups. (B-G) MMP12, MM13, CCL2 and Kv1.3 were upregulated by MgTX in the M2 phenotype groups; however, POSTN and δ-catenin were downregulated by MgTX in the M2 phenotype groups. No significant difference was observed between the control (N) and MgTX groups. *P<0.05 vs. M1 group; #P<0.05 vs. M2 group. n=3. MgTX, margatoxin; HF, hepatic fibrosis; MMP, matrix metalloproteinase; POSTN, periostin; CCL2, C-C motif chemokine ligand 2.