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. 2019 Nov 13;45(1):162–174. doi: 10.3892/ijmm.2019.4404

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Copyright: © Wu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effects of APP overexpression and silencing on the expression and phosphorylation levels of MAPK signaling pathway-related factors in MDA-MB-231 breast cancer cells. (A) Protein expression and phosphorylation levels of MAPK signaling pathway-related factors following APP overexpression and silencing. (B) mRNA expression level of MAPK signaling pathway-related molecules by RT-qPCR following APP overexpression and silencing. (C) Statistical analysis of the western blot bands. APP overexpression significantly increased the phosphorylation levels of MLK3, MEK4 and JNK3 in MDA-MB-231 breast cancer cells, when compared with the control groups. All experiments were performed in triplicate. Data are presented as the mean ± SD. ^*P<0.05 vs. control. APP, amyloid precursor protein; MLK3, mixed lineage kinase 3; MEK4, mitogen-activated protein kinase kinase 4; t-, total; p-, phosphorylated; shRNA, short hairpin RNA; MAPK, mitogen-activated protein kinase.