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. 2019 Nov 13;45(1):195–209. doi: 10.3892/ijmm.2019.4407

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Copyright: © Gao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Rapamycin promoted the basal and angiotensin II-induced H9c2 cell autophagy in the 30 min rapamycin pretreatment groups. (A) Autophagy was assessed by western blot of light chain 3B II, p62 and Beclin-1 in H9c2 cells. (B) Immunofluorescence staining for autophagosomes in H9c2 cells, which was marked by Alexa Fluor 488-conjugated light chain 3B (green). The nuclei were stained by 4′,6-diamidino-2-phenylindole (blue). ^*P<0.05 vs. the corresponding control group; ^#P<0.05 vs. the corresponding angiotensin II-treated group. Ang II, angiotensin II; Rap, rapamycin; DAPI, 4′,6-diamidino-2-phenylindole.

Rapamycin promoted the basal and angiotensin II-induced H9c2 cell autophagy in the 30 min rapamycin pretreatment groups. (A) Autophagy was assessed by western blot of light chain 3B II, p62 and Beclin-1 in H9c2 cells. (B) Immunofluorescence staining for autophagosomes in H9c2 cells, which was marked by Alexa Fluor 488-conjugated light chain 3B (green). The nuclei were stained by 4′,6-diamidino-2-phenylindole (blue). ^*P<0.05 vs. the corresponding control group; ^#P<0.05 vs. the corresponding angiotensin II-treated group. Ang II, angiotensin II; Rap, rapamycin; DAPI, 4′,6-diamidino-2-phenylindole.