Figure 1.

Effects of hypoxia on the proliferation of HMC-1 cells. (A) Following culture under hypoxic conditions, the expression of HIF-1α in HMC-1 cells was determined by western blotting and used to confirm that cells exist within a hypoxic environment. (B) The proliferation of HMC-1 cells under hypoxic and normoxic conditions was examined by the Cell Counting Kit-8 assay for the indicated time periods. There were 4 biological replicates/condition. Error bars represent the standard deviation. ^*P<0.05, ^**P<0.01. HMC-1, human mast cells; HIF-1α, hypoxia inducible factor-1α; OD, optical density.